Composite

Part:BBa_K2496002:Design

Designed by: Kang, jin-wook   Group: iGEM17_TNCR_Korea   (2017-10-27)


strong DPP4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 791
    Illegal PstI site found at 1565
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1781
    Illegal NheI site found at 2282
    Illegal PstI site found at 791
    Illegal PstI site found at 1565
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2208
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 791
    Illegal PstI site found at 1565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 791
    Illegal PstI site found at 1565
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 702
    Illegal BsaI site found at 2069


Design Notes

DPP4 sequence have a two Pst1 site. So, we need to know about new ligation method to move the DPP4 into the pSBIC3.


Source

Mouse DPP4 gene was acquired from the Korea gene bank.

References